KMID : 1001920150570050329
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Journal of Korean Neurosurgical Society 2015 Volume.57 No. 5 p.329 ~ p.334
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Overexpressions of Vimentin and Integrins in Human Metastatic Spine Tumors
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Park Sung-Bae
Ryu Young-Joon Chung Young-Seob Kim Chi-Heon Chung Chun-Kee
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Abstract
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Objective: To comparatively investigate the expression of several integrins in specimens of human bone metastases and degenerative bone tissue.
Methods: Degenerative cancellous tissue was obtained from a sample of human degenerative spine. Thirteen human specimens were obtained from metastatic spine tumors, whose primary cancer was colon cancer (n=3), hepatocellular cancer (n=3), lung cancer (n=4), and breast cancer (n=3). The expression of vimentin and integrins ¥áv, ¥â1, and ¥â3 was assessed in metastatic and degenerative specimens by immunohistochemistry and real-time reverse transcription-polymerase chain reaction (qRT-PCR).
Results: Immunohistochemical staining showed that vimentin and integrin ¥áv was broadly expressed in all tissues examined. By contrast, integrin ¥â1 was weakly expressed only in 38.4% (5/13) of tissues. Integrin ¥â3 was consistently negative in all cases examined. qRT-PCR analysis showed that vimentin gene expression was higher in all metastatic specimens, as compared to degenerative bone. The gene expression of integrin ¥áv in breast specimen was significantly higher than others (p=0.045). The gene expression of integrin ¥â1 was also higher in all metastatic specimens than in degenerative bone tissue. The gene expression of integrin ¥â3 was variable.
Conclusion: Spinal metastatic tumors have mesenchymal characteristics such as increased expression of vimentin. The increased expression of integrin ¥áv and ¥â1 in spine metastatic tumors suggests that adhesive molecules such as integrin may have implications for the prevention of spine metastasis.
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KEYWORD
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Spine, Metastasis, Integrin ¥áv, Vimentin
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